Sex-specific neuronal cultures from cryopreserved preparations

The NIH now formally mandates that all grant-funded research address Sex as a Biological Variable (SABV), a requirement that strictly applies to studies using primary neuronal cultures. Because these cultures are typically prepared from fetal rodent brain tissue, where sex cannot be determined visually, researchers are forced to genotype the fetuses via PCR. This genotyping step drastically extends the time and labor required to prepare the cultures.

This tedious workflow would be completely eliminated if cryopreserved, sex-specific neurons were commercially available. However, because no such products currently exist on the market, Spot Cells LLC was awarded a Phase I SBIR grant from the NIH to solve this problem. Utilizing our proprietary technology, we successfully generated and validated sex-specific primary cultures of mouse cortical neurons. Images tracking the exact same microscopic field of male and female neurons from Day-In-Vitro (DIV) 1 through 6 are shown above.

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